Part:BBa_K1891015
P(3HB)(BBa_K934001)+HSP promotor (BBa_K873002)
Poly-3-hydroxybutyrate, P(3HB), a kind of PHAs is synthesized by three enzymes. The A gene encodes for the 393 amino acids protein, 3-ketothiolase (PhaA). The B1 gene encodes for the 246 amino acids protein, acetoacetyl-CoA reductase (PhaB). The C1 gene encodes for the 589 amino acids protein, PHA Synthase (PhaC). These three enzymes can use Co-A as a substrate to produce P(3HB). IGEM team Tokyo Tech2012 designed and synthesized phaC1-A-B1gene sequence BBa_K934001, and expressed via constitutive promoter. In order to make the production process more controllable, we added heat-sensitive promoter(BBa_K873002) to the upstream of phaC1-A-B1 gene sequence （BBa_K934001）, constructing the heatshock inducible parts （BBa_K1891015). Then we linked the newly synthesized part to the standard vector pSB1C3. The new plasmids can be used directly for heatshock induced expression in E.coli.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 916
Illegal BglII site found at 1741 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 222
Illegal NgoMIV site found at 293
Illegal NgoMIV site found at 893
Illegal NgoMIV site found at 1205
Illegal NgoMIV site found at 1484
Illegal NgoMIV site found at 2136
Illegal NgoMIV site found at 2158 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 4002
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